Milo™ is the world's first Single-Cell Western platform. It measures protein expression in thousands of single cells in a single run so the user can profile heterogeneity in her samples. A cell suspension is loaded and the Sc West chip captures ~1,000 single cells. Milo then does a fast, 1-minute SDS-PAGE separation on each single-cell lysate on-chip. Probing with the user's favorite conventional Western antibodies can measure ~12 proteins per cell using a variety of multiplexing strategies. Single-Cell Westerns can be used to unlock the single-cell proteome and measure more of the proteome than is possible with any other single-cell technique.
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