RNAscope® 2.5 HD Duplex Assay

RNA in situ expression of CD274 (green) and CD8A (red) in human lung cancer FFPE tissue using RNAscope® 2.5 HD Duplex Assay

  • This assay can be used for simultaneous visualization of two RNA targets while maintaining single cell resolution.
  • The applications of this assay include study of gene co-regulation, profile gene expression in a specific cell type (identified using a cell specific marker) and map coexpression of two targets within the same cellular context based on reliable chromogenic detection of the two transcripts.

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